<ul class="tab-inside"><li><p style="text-align:justify;"><span style="font-family:Arial,Helvetica;">Small peptide binding stiffens the ubiquitin-like protein SUMO1&nbsp;</span><br><span style="font-family:Arial,Helvetica;">Hema Chandra Kotamarthi, Anju Yadav, and Sri Rama Koti Ainavarapu*&nbsp;</span><br><span style="font-family:Arial,Helvetica;">Biophysical Journal, Vol 108, No 2 (2015) p360-367.</span></p></li><li><p style="text-align:justify;"><span style="font-family:Arial,Helvetica;">Mechanical Unfolding of Ribose Binding Protein (RBP) and Its Comparison with Other&nbsp;</span><br><span style="font-family:Arial,Helvetica;">Periplasmic Binding Proteins (PBPs)&nbsp;</span><br><span style="font-family:Arial,Helvetica;">Hema Chandra Kotamarthi, Satya Narayan, and Sri Rama Koti Ainavarapu*&nbsp;</span><br><span style="font-family:Arial,Helvetica;">Journal of Physical Chemistry B, Vol 118 (2014), No 39, p11449-11454.</span></p></li><li><p style="text-align:justify;"><span style="font-family:Arial,Helvetica;">Ca2+ binding enhanced mechanical stability of an archaeal crystallin&nbsp;</span><br><span style="font-family:Arial,Helvetica;">Venkatraman Ramanujam, Hema Chandra Kotamarthi, Sri Rama Koti Ainavarapu*&nbsp;</span><br><span style="font-family:Arial,Helvetica;">PLOS ONE Vol 9, (2014), e94513</span></p></li><li><p style="text-align:justify;"><span style="font-family:Arial,Helvetica;">Preventing Disulphide Bond Formation Weakens Non-covalent Forces Among Lysozyme Aggregates&nbsp;</span><br><span style="font-family:Arial,Helvetica;">Vijay Kumar Ravi, Mohit Goel, Hema Chandra Kotamarthi, Sri Rama Koti Ainavarapu, and Rajaram Swaminathan*&nbsp;</span><br><span style="font-family:Arial,Helvetica;">PLOS ONE, Vol 9 (2014), e87012</span></p></li><li><span style="font-family:Arial,Helvetica;">Multiple unfolding pathways of leucine binding protein (LBP) probed by single-molecule force spectroscopy (SMFS)&nbsp;</span><br><span style="font-family:Arial,Helvetica;">Hema Chandra Kotamarthi, Riddhi Sharma, Satya Narayan, Sayoni Ray, and Sri Rama Koti Ainavarapu*&nbsp;</span><br><span style="font-family:Arial,Helvetica;">J. Am. Chem. Soc. Vol 135 (2013) p14768-14774&nbsp;</span><br><span style="font-family:Arial,Helvetica;">This paper is highlighted in ‘Spotlights on Recent JACS Publications’ of JACS,&nbsp;</span><br><span style="font-family:Arial,Helvetica;">2013, 135 (41), pp 15269, : Pulling on a Protein To Map Its Unfolding Pathways</span></li><li>Single-molecule studies on polySUMO proteins reveal their mechanical flexibility, Hema Chandra Kotamarthi, Riddhi Sharma, and Sri Rama Koti Ainavarapu<sup>*</sup> Biophysical Journal Vol. 104, No. 10 (2013) p 2273-2281.</li><li>Iterative cloning, overexpression, purification and isotopic labeling of an engineered dimer of a Ca<sup>2+</sup>-binding protein of the bg-crystallin superfamily from Methanosarcina acetivorans<br>Venkatraman Ramanujam, Kandala V.R. Chary, and Sri Rama Koti Ainavarapu*<br>Protein Expression and Purification Vol 84 (2012) p 116-122.</li><li>Ligand modulated parallel mechanical unfolding pathways of Maltose Binding Proteins (MBPs),<br>Vasudha Aggarwal, S. Rajendra Kulothungan, M.M. Balamurali, S.R. Saranya, Raghavan Varadarajan, and Sri Rama Koti Ainavarapu,<br>J. Biol.Chem. Vol 286, No. 32 (2011) p28056-28065.</li><li>Single-molecule force-clamp spectroscopy: Probing transition states of mechanically activated chemical reactions,<br>Sri Rama Koti Ainavarapu,<br>ISRAPS Bulletin Vol. 21 (2009) p32-39.</li><li>A Single-Molecule Perspective on the Role of Solvent Hydrogen Bonds in Protein Folding and Chemical Reactions,<br>Lorna Dougan, Sri Rama Koti Ainavarapu, Georgi Genchev, Hui Lu and Julio M. Fernandez,<br>Chem Phys Chem Vol. 9 (2008) p2836-2847.</li><li>Single-Molecule Force Spectroscopy Measurements of Bond Elongation during a Bimolecular Reaction,<br>Sri Rama Koti Ainavarapu, Arun P. Wiita, Lorna Dougan, Einar Uggerud, and Julio M. Fernandez,<br>Journal of American Chemical Society Vol. 130 (2008) p6479-6487. (Our mechanochemistry JACS paper has been mentioned in Research Highlights section of Nature (2008), 453, p261: “Disulphide dichotimies”.)</li><li>A Single-Molecule Assay to Directly Identify Solvent Accessible Disulfide Bonds and Probe Their Effect on Protein Folding<br>Sri Rama Koti Ainavarapu*, Arun P. Wiita, Hector H. Huang, and Julio M. Fernandez<br>Journal of American Chemical Society Vol. 130 (2008) p436-437.</li><li>Contour Length and Refolding Rate of a Small Protein Controlled by Engineered Disulfide Bonds<br>Sri Rama Koti Ainavarapu, Jasna Brujic, Hector H. Huang, Arun P. Wiita, Hui Lu, Lewyn Li, Kirstin A. Walther, Mariano Carrion-Vazquez, Hongbin Li, and J.M. Fernandez<br>Biophysical Journal Vol. 92, No. 1 (2007) p225-233.</li><li>Mechanical Unfolding Pathways of the Enhanced yellow Fluorescent Protein Revealed by Single Molecule Force Spectroscopy<br>Raul Perez-Jimenez, Sergi Garcia-Manyes, Sri Rama Koti Ainavarapu, and J.M. Fernandez<br>J. Biol.Chem. Vol. 281, No. 52 (2006) p40010-40014.</li><li>Force-dependent chemical kinetics of disulfide bond reduction observed with single-molecule techniques<br>Arun P. Wiita, Sri Rama Koti Ainavarapu, Hector H. Huang, and J.M. Fernandez<br>Proc. Natl. Acad. Sci. (USA) Vol. 103, No. 19 (2006) p7222-7227. (Commentary on this article is reported in PNAS (2006), 103, p7533: “Covalent chemistry on distended proteins”)</li><li class="last">Ligand binding modulates the mechanical stability of dihydrofolate reductase (DHFR),&nbsp;<br>Sri Rama Koti Ainavarapu, Lewyn Li, Carmen L. Badilla, and J.M. Fernandez,<br>Biophysical Journal Vol. 89, No. 5 (2005) p3337-3344.</li></ul>