<h3>Title</h3>
<p><span style="color: #0000ff;">From trees to forests: studying biochemical machines in the broader context</span></p>
<p><strong><span style="text-decoration: underline;">Abstract</span> :</strong></p>
<p style="text-align: justify;">In this talk, I will discuss future directions of investigating the heterogeneities and complexities intranscription mechanisms. I will first show how differences in promoter sequence elements lead toalteration of entire mechanistic pathways, highlighting the possibility of massive variations in transcriptionkinetics and gene expression patterns due to subtle changes in an important determinant. Building on thisobservation, I would outline strategies for developing massively parallel single-molecule assays tointerrogate sets of promoters with randomized sequence elements and explore transcription mechanismsacross the promoter space. I would also discuss proposals for monitoring conformational changes in a livebacterium to enable mechanistic studies of biochemical reactions in a physiological setting and underchanging environmental conditions. Finally, I will discuss plans to employ these techniques to study themechanism of coupling between the transcription and translation machinery – a process that underpinsgene regulation in bacteria.</p>