<h3>Title</h3>
<h3>Regioselective, stereoselective cyclopropanation of structurally diverse alkenes</h3>
<p><strong><span style="text-decoration: underline;">Abstract</span> :</strong></p>
<p>Cytochrome P450known for C-H activation to C-OH. According to previous literature, Ir is the best in place of the Fe in cytochrome p450 for cyclopropanation. At first, I have taken out the heme group using Teale’s method and reconstituted Ir(por) in apo CYP175A1. We have seen 100% removal of the heme group and 50% reconstituted Ir(por)CYP175A1 concentration of ~2uM (according to ICPMS data). Then, we carried out cyclopropanation of (R)-(+)-limonene, using ethyl diazo acetate (EDA) (slow addition) at 40<sup>o</sup>C in an N2 atmosphere. We have seen the formation of a product RT 5.08 with Ir(por)CYP175A1 which is very similar to Ir(por) control.</p>
<p>Then, we shifted to surfactants like CTAB and DTAB micelles formation are conformed by DLS. Then we have carried out Limonene, Ir(por), and EDA reactions in a confined space of micelles. CTAB shows promising results in GC-FID with two new peaks at RT 4.83 and RT 4.89. Our GC-MS was down, hence, reverse phase HPLC coupled with UV-Vis (wavelength taken 210 nm, 225 nm, 240 nm, 250 nm) was carried out, and additional peaks were observed at A (RT 4.15), B (RT 4.35), C (RT 4.55), and D (RT 5.15) in the case of DTAB and CTAB. The area under the curve of the peaks observed that all peaks are reproducible and increase with time (24hrs to 36hr to 48hrs). We have analyzed these peaks using ESI-MS. ESI-MS of peak A observed a mass of 309.20 which corresponds to the dicyclopropanated derivative of Limonene and peak D observed a mass of 223.17 corresponding to the monocyclopropanated derivative of Limonene. ESI-MS peaks of C and D are mixed compounds. More product yields have been observed in shorter times (24 and 36hrs) may be due to the higher solubility of limonene in the hydrophobic core of CTAB. A high product yield in a longer period (48hr) in the case of DTAB may be due to more solubilized EDA.</p>